Autoantigen arrays for multiplex analysis of antibody isotypes.
نویسندگان
چکیده
We describe here a microarray-based method for multiplexed, antigen-specific assessment of immunoglobulin (Ig) subclasses. We used 1152-feature arrays composed of 140 antigens or antigen fragments to detect isotype-specific mAb, to quantitatively monitor changes in isotype mAb concentration, and to profile antigen-specific antibody isotype production in a murine model of autoimmunity. This platform can be easily adapted to a variety of applications, and has the potential to elucidate mechanisms that govern development and evolution of antibody responses in in vivo and in vitro systems.
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عنوان ژورنال:
- Proteomics
دوره 6 21 شماره
صفحات -
تاریخ انتشار 2006